Experimental Zooprophylactic Institute of southern Italy, via Salute, 2, 80055, Portici, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", via Vivaldi - 43, 81100 Caserta, Italy.
Biomolecules. 2020 May 19;10(5):788. doi: 10.3390/biom10050788.
The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene encoding a LuxR-like regulator repressing genes. In this study, we show for the first time the ability of MucR to bind the promoter of in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate that MucR can bind the gene promoter. Analyses by RT-qPCR showed no significant differences in the expression level of genes in CC092 lacking MucR compared to the wild-type strain, indicating that MucR binding to the promoter has little impact on gene expression in 2308. The MucR modality to bind the two promoters analyzed supports our previous hypothesis that this is a histone-like protein never found before in .
布鲁氏菌属 abortus 的蛋白 MucR 被描述为许多毒力基因的转录调节剂。它是 Ros/MucR 家族的成员，该家族包含控制与真核宿主成功相互作用的 α-变形菌的重要基因表达的蛋白质。尽管有明确的证据表明 MucR 在抑制毒力基因方面的作用，但到目前为止，还没有研究表明这种蛋白质与它的靶基因启动子之间存在直接相互作用，该靶基因编码一种抑制基因的 LuxR 样调节蛋白。在这项研究中，我们首次展示了 MucR 在电泳迁移率变动分析中结合 的能力，证明了 MucR 在抑制该基因方面的直接作用。此外，我们证明 MucR 可以结合 基因的启动子。RT-qPCR 分析显示，缺失 MucR 的 CC092 与野生型 菌株相比， 基因的表达水平没有显著差异，这表明 MucR 结合 启动子对 基因在 2308 中的表达影响很小。分析表明，MucR 结合这两个启动子的方式支持我们之前的假设，即这是一种以前从未在 中发现的组蛋白样蛋白。
