研究基质成分在保护洋葱伯克霍尔德菌复合体生物膜对抗妥布霉素中的作用。
Investigating the role of matrix components in protection of Burkholderia cepacia complex biofilms against tobramycin.
机构信息
Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
出版信息
J Cyst Fibros. 2014 Jan;13(1):56-62. doi: 10.1016/j.jcf.2013.07.004. Epub 2013 Aug 8.
BACKGROUND
Burkholderia cepacia complex (Bcc) organisms produce a wide variety of potential virulence factors, including exopolysaccharides (EPS), and exhibit intrinsic resistance towards many antibiotics. In the present study we investigated the contribution of Bcc biofilm matrix components, including extracellular DNA, cepacian and poly-β-1,6-N-acetylglucosamine, to tobramycin susceptibility.
METHODS
The in vitro bactericidal activity of tobramycin in combination with recombinant human DNase (rhDNase), NaClO and dispersin B was tested against Bcc biofilms.
RESULTS
EPS degradation by NaClO pretreatment and specific PNAG degradation by dispersin B significantly increased the bactericidal effect of tobramycin towards some of the Bcc biofilms tested, including the strains of Burkholderia cenocepacia, B. cepacia and Burkholderia metallica. The presence of rhDNase during biofilm treatment and/or development had no influence on tobramycin activity.
CONCLUSION
These results suggest that EPS play a role in tobramycin susceptibility of Bcc biofilms and that matrix degrading combination therapy could improve treatment of Bcc biofilm infections.
背景
洋葱伯克霍尔德菌复合群（Bcc）产生多种潜在的毒力因子，包括胞外多糖（EPS），并对许多抗生素表现出固有耐药性。在本研究中，我们研究了 Bcc 生物膜基质成分（包括细胞外 DNA、cepacian 和聚-β-1,6-N-乙酰葡糖胺）对妥布霉素敏感性的贡献。
方法
用重组人 DNA 酶（rhDNase）、次氯酸钠（NaClO）和分散素 B 检测妥布霉素与体外 Bcc 生物膜的联合杀菌活性。
结果
NaClO 预处理降解 EPS 以及分散素 B 特异性降解 PNAG 显著增加了妥布霉素对一些测试的 Bcc 生物膜的杀菌作用，包括洋葱伯克霍尔德菌、鲍曼不动杆菌和金属伯克霍尔德菌。在生物膜处理和/或发育过程中添加 rhDNase 对妥布霉素活性没有影响。
结论
这些结果表明 EPS 在 Bcc 生物膜对妥布霉素的敏感性中起作用，基质降解联合治疗可能改善 Bcc 生物膜感染的治疗。
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