通过增加组氨酸供应来增加奥利格酵母中硬脂烯酸。
Increase in stearidonic acid by increasing the supply of histidine to oleaginous Saccharomyces cerevisiae.
机构信息
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
出版信息
J Biosci Bioeng. 2014 Jan;117(1):53-6. doi: 10.1016/j.jbiosc.2013.06.004. Epub 2013 Aug 6.
Increasing concentration of histidine significantly increased stearidonic acid production and cell growth in oleaginous Saccharomyces cerevisiae that has been genetically modified by Δsnf2 disruption, DGA1 and Δ6 desaturase gene overexpression, and LEU2 expression. High concentration of histidine in wild-type transformant and HIS3 expression in Δsnf2 transformant also increased stearidonic acid.
增加组氨酸的浓度显著提高了通过 Δsnf2 敲除、DGA1 和 Δ6 去饱和酶基因过表达以及 LEU2 表达进行遗传修饰的产油酿酒酵母的硬脂烯酸产量和细胞生长。在野生型转化体中高浓度的组氨酸和 Δsnf2 转化体中的 HIS3 表达也增加了硬脂烯酸。
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