Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, Shandong, China.
Departmento de Genética y Microbiología (Unidad Asociada al Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas), Facultad de Biología, Universidad de Murcia, Murcia 30100, Spain.
Int J Mol Sci. 2019 Apr 4;20(7):1683. doi: 10.3390/ijms20071683.
Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of its nutritional value and pharmaceutical applications. is a GLA producing filamentous fungus, which can be a useful tool to produce SDA. This study has, therefore, overexpressed the () gene from in this fungus to construct a SDA-producing cell factory. To produce SDA in , the homologous overexpression of gene was analyzed. When the gene was overexpressed in CBS 277.49, up to 5.0% SDA was accumulated in this strain. According to current knowledge, this is the first study describing the construction of a SDA-producing cell factory by overexpression of gene in oleaginous fungus . A new scope for further research has been established by this work to improve SDA production in this fungus, specifically in its high lipid-producing strain, WJ11.
硬脂酸（SDA；18:4，n-3）是γ-亚麻酸（GLA；18:3，n-6）的δ15-去饱和酶产物和α-亚麻酸（ALA；18:3，n-3）的δ6-去饱和酶产物。构建工程化产油微生物来生产 SDA 引起了人们的极大兴趣，因为它具有营养价值和药用应用。 是一种产生 GLA 的丝状真菌，它可以作为生产 SDA 的有用工具。因此，本研究在该真菌中过表达了来自 的 () 基因，以构建 SDA 生产细胞工厂。为了在 中生产 SDA，分析了 基因的同源过表达。当该基因在 CBS 277.49 中过表达时，该菌株中积累了高达 5.0%的 SDA。根据目前的知识，这是首次描述在产油真菌中通过过表达 基因构建 SDA 生产细胞工厂的研究。这项工作为进一步提高该真菌中 SDA 的产量，特别是在其高产脂菌株 WJ11 中，开辟了新的研究领域。
