Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya'an, Sichuan 625014, PR China.
Gene. 2013 Oct 25;529(2):332-9. doi: 10.1016/j.gene.2013.07.104. Epub 2013 Aug 7.
CD8 molecule is a cell membrane glycoprotein, which plays an important role in cell-mediated immunity. Here, we identified Chinese goose CD8α (goCD8α) gene for the first time. The full-length cDNA of goCD8α is 1459bp in length and contains a 711bp open reading frame. Phylogenetic analysis shows that the waterfowl CD8α formed a monophyletic group. Semi-quantitative RT-PCR analysis showed that transcripts of goCD8α mRNA were high in the immune-related organs and mucosal immune system in gosling, and high in thymus and spleen comparing to other immune-related tissues in goose. The obvious increase of CD8α expression was observed in spleen of acute new type gosling viral enteritis virus (NGVEV) infected bird, while the increase of CD8α were observed in the thymus, bursa of fabricius, and cecum of chronic infected bird. The CD8α mRNA transcription level in spleen mononuclear cells was significantly up-regulated when stimulated by phytohemagglutinin, but not by lipopolysaccharide in vitro.
CD8 分子是一种细胞膜糖蛋白，在细胞介导的免疫中发挥重要作用。在这里，我们首次鉴定了中国鹅 CD8α（goCD8α）基因。goCD8α 的全长 cDNA 长 1459bp，包含一个 711bp 的开放阅读框。系统进化分析表明，水禽 CD8α 形成了一个单系群。半定量 RT-PCR 分析显示，goCD8αmRNA 转录本在雏鹅的免疫相关器官和黏膜免疫系统中高表达，在鹅的其他免疫相关组织中，胸腺和脾脏中的表达量较高。在急性新型鹅细小病毒肠炎病毒（NGVEV）感染的鸟类的脾脏中观察到 CD8α 表达明显增加，而在慢性感染的鸟类的胸腺、法氏囊和盲肠中也观察到 CD8α 的增加。在体外，植物血凝素刺激后，脾单核细胞中的 CD8αmRNA 转录水平显著上调，但脂多糖没有上调。
