Ochiai K, Iwamoto I, Kimura A, Nakagawa N, Tomioka H, Yoshida S, Takahashi H, Yoshida A
Second Department of Internal Medicine, Chiba University School of Medicine.
Arerugi. 1990 May;39(5):459-64.
To investigate the heterogeneity of human eosinophils, we established a murine anti-human eosinophil monoclonal antibody AE500 (IgM, kappa) by immunizing blood eosinophils from patients with idiopathic eosinophilia. The reactivity of AE500 with human leukocytes was analyzed by a fluorescence-activated cell sorter. AE500 reacted with all the populations of blood eosinophils and neutrophils from patients with eosinophilia (idiopathic eosinophilia, n X 4 or asthma, n =), but not with those from asthmatic patients without eosinophilia (n = 8) or normal subjects (n = 6). AE500 did not react with granulocytes from other family members of AE500-positive individuals. These results suggest that the anti-human eosinophil antibody AE500 reacts with a cell surface antigen of blood granulocytes in a hypereosinophilic state. This anti-eosinophilic antibody would be useful in investigating the mechanism of eosinophilia in a variety of diseases.
为了研究人类嗜酸性粒细胞的异质性，我们通过用特发性嗜酸性粒细胞增多症患者的血液嗜酸性粒细胞进行免疫，制备了一种鼠抗人嗜酸性粒细胞单克隆抗体AE500（IgM，κ）。通过荧光激活细胞分选仪分析AE500与人白细胞的反应性。AE500与嗜酸性粒细胞增多症患者（特发性嗜酸性粒细胞增多症，n = 4或哮喘，n =）的所有血液嗜酸性粒细胞和中性粒细胞群体发生反应，但与无嗜酸性粒细胞增多症的哮喘患者（n = 8）或正常受试者（n = 6）的细胞不发生反应。AE500不与AE500阳性个体的其他家庭成员的粒细胞发生反应。这些结果表明，抗人嗜酸性粒细胞抗体AE500与处于嗜酸性粒细胞增多状态的血液粒细胞的细胞表面抗原发生反应。这种抗嗜酸性粒细胞抗体将有助于研究各种疾病中嗜酸性粒细胞增多的机制。
