Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, T4000ILC Tucumán, Argentina.
Meat Sci. 2014 Jan;96(1):295-303. doi: 10.1016/j.meatsci.2013.07.010. Epub 2013 Jul 18.
The ability of meat borne anti-Listeria Lactobacillus to form biofilms under different in vitro conditions and on abiotic surfaces was investigated. Biofilm formation by the adhesion to polystyrene microtiter plates was determined, this being higher for Lactobacillus curvatus CRL1532 and CRL705 and Lactobacillus sakei CRL1862. The physicochemical properties of the cell surface were relatively hydrophilic and acidic in character; L. sakei CRL1862 exhibiting the strongest autoaggregation. The adhesion of lactobacilli to stainless steel (SS) and polytetrafluoroethylene (PTFE) supports at 10°C was found to be maximal for L. sakei CRL1862 on SS after 6 days. When biofilm architecture was characterized by epifluorescence and SEM, L. sakei CRL1862 homogeneously covered the SS surface while cell clusters were observed on PTFE; the extracellular polymeric substance matrix adapted to the topography and hydrophilic/hydrophobic characteristics of each material. The feasibility of L. sakei CRL1862 to form biofilm on materials used in meat processing highlights its potential as a control strategy for Listeria monocytogenes biofilms.
研究了肉源抗李斯特菌乳杆菌在不同体外条件下和非生物表面形成生物膜的能力。通过粘附聚苯乙烯微量滴定板来确定生物膜的形成，其中弯曲乳杆菌 CRL1532 和 CRL705 以及清酒乳杆菌 CRL1862 的生物膜形成能力更高。细胞表面的物理化学性质具有相对亲水性和酸性；清酒乳杆菌 CRL1862 表现出最强的自聚集。发现乳杆菌在 10°C 下对不锈钢（SS）和聚四氟乙烯（PTFE）的粘附在 6 天后对 SS 上的清酒乳杆菌 CRL1862 达到最大值。当通过荧光和 SEM 对生物膜结构进行表征时，清酒乳杆菌 CRL1862 均匀地覆盖 SS 表面，而在 PTFE 上观察到细胞簇；细胞外聚合物基质适应每种材料的形貌和亲水/疏水特性。清酒乳杆菌 CRL1862 在肉类加工中使用的材料上形成生物膜的可行性突出了其作为李斯特菌生物膜控制策略的潜力。
