Chavant Patrick, Martinie Brigitte, Meylheuc Thierry, Bellon-Fontaine Marie-Noëlle, Hebraud Michel
Station de Recherches sur la Viande-Microbiologie, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genès Champanelle, France.
Appl Environ Microbiol. 2002 Feb;68(2):728-37. doi: 10.1128/AEM.68.2.728-737.2002.
The surface physicochemical properties of Listeria monocytogenes LO28 under different conditions (temperature and growth phase) were determined by use of microelectrophoresis and microbial adhesion to solvents. The effect of these parameters on adhesion and biofilm formation by L. monocytogenes LO28 on hydrophilic (stainless steel) and hydrophobic (polytetrafluoroethylene [PTFE]) surfaces was assessed. The bacterial cells were always negatively charged and possessed hydrophilic surface properties, which were negatively correlated with growth temperature. The colonization of the two surfaces, monitored by scanning electron microscopy, epifluorescence microscopy, and cell enumeration, showed that the strain had a great capacity to colonize both surfaces whatever the incubation temperature. However, biofilm formation was faster on the hydrophilic substratum. After 5 days at 37 or 20 degrees C, the biofilm structure was composed of aggregates with a three-dimensional shape, but significant detachment took place on PTFE at 37 degrees C. At 8 degrees C, only a bacterial monolayer was visible on stainless steel, while no growth was observed on PTFE. The growth phase of bacteria used to inoculate surfaces had a significant effect only in some cases during the first steps of biofilm formation. The surface physicochemical properties of the strain are correlated with adhesion and surface colonization.
利用微电泳和微生物对溶剂的粘附作用，测定了不同条件（温度和生长阶段）下单增李斯特菌LO28的表面物理化学性质。评估了这些参数对单增李斯特菌LO28在亲水性（不锈钢）和疏水性（聚四氟乙烯[PTFE]）表面上的粘附和生物膜形成的影响。细菌细胞始终带负电荷，并具有亲水性表面性质，这与生长温度呈负相关。通过扫描电子显微镜、落射荧光显微镜和细胞计数监测的两个表面的定殖情况表明，无论培养温度如何，该菌株都具有在两个表面定殖的强大能力。然而，生物膜在亲水性基质上形成得更快。在37℃或20℃下培养5天后，生物膜结构由三维形状的聚集体组成，但在37℃时PTFE上发生了显著的脱落。在8℃时，不锈钢上仅可见细菌单层，而在PTFE上未观察到生长。用于接种表面的细菌生长阶段仅在生物膜形成的最初步骤中的某些情况下具有显著影响。该菌株的表面物理化学性质与粘附和表面定殖相关。
