Unidad de Recursos Forestales, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, 50059, Zaragoza, Spain.
Tree Physiol. 2013 Jul;33(7):695-700. doi: 10.1093/treephys/tpt052.
The turgor loss point (TLP), which is considered a threshold for many physiological processes, may be useful in plant-breeding programs or for the selection of reforestation species. Obtaining TLP through the standard pressure-volume (p-v) curve method in a large set of species is highly time-consuming and somewhat subjective. To solve this problem, we present an objective and a less time-consuming technique based on the leaf resonance able to calculate the relative water content (RWC) at TLP (RWCTLP). This method uses air-coupled broadband ultrasonic spectroscopy to obtain the sigmoidal relation between RWC and the standardized resonant frequency (f/fo). For the 13 species measured, the inflexion point of the RWC-f/fo relationship ( ) was not statistically different from the value of RWC at the TLP obtained with the p-v curves (RWCTLP p-v).
膨压损失点（TLP）被认为是许多生理过程的一个阈值，它可能对植物育种计划或造林物种的选择有用。在大量物种中通过标准的压力-体积（p-v）曲线法获得 TLP 非常耗时且有些主观。为了解决这个问题，我们提出了一种基于叶片共振的客观且耗时更少的技术，能够计算 TLP 时的相对含水量（RWC）（RWCTLP）。该方法使用空气耦合宽带超声光谱获得 RWC 与标准化共振频率（f/fo）之间的 S 形关系。在所测量的 13 个物种中，RWC-f/fo 关系的拐点（ ）与通过 p-v 曲线获得的 TLP 时的 RWC 值（RWCTLP p-v）在统计学上没有差异。
