Freudenstein J, Wagner S, Luck R M, Einspanier R, Scheit K H
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, W. Germany.
Biochem Biophys Res Commun. 1990 Aug 31;171(1):250-6. doi: 10.1016/0006-291x(90)91384-5.
A cDNA library derived from poly(A+)RNA of bovine ovary was screened with a PCR fragment comprising the coding region of human tissue inhibitor of metalloproteinase inhibitor (TIMP). From a number of positive clones, pBGR19, containing a 747 bp insert, was identified and sequenced. The derived amino acid sequence represents that of the precursor of bovine TIMP. Northern analysis reveals a TIMP specific mRNA of 800 bp. Southern analysis indicates that one gene appears to specify bovine TIMP. TIMP mRNA is only weakly expressed in follicular granulosa- and theca cells, whereas luteinization of the follicle is associated with an increase of expression. Expression varies with the stage of the luteal phase; it was highest in stages I and III, but low in stages II and IV of the oestrous cycle.
用人金属蛋白酶抑制剂（TIMP）编码区的PCR片段筛选从牛卵巢的聚腺苷酸（poly(A+)）RNA构建的cDNA文库。从多个阳性克隆中鉴定出含747 bp插入片段的pBGR19并进行测序。推导的氨基酸序列代表牛TIMP前体的序列。Northern分析显示一条800 bp的TIMP特异性mRNA。Southern分析表明一个基因似乎决定牛TIMP。TIMP mRNA在卵泡颗粒细胞和卵泡膜细胞中仅微弱表达，而卵泡的黄体化与表达增加有关。表达随黄体期阶段而变化；在发情周期的I期和III期最高，但在II期和IV期较低。
