Nara Institute of Science and Technology, Graduate School of Information Science, Nara, Japan 630-0192.
J Biomed Opt. 2013 Nov;18(11):111411. doi: 10.1117/1.JBO.18.11.111411.
A simple optical tweezers design is proposed to manipulate particles in the axial direction and estimate particle position with nanometer sensitivity. Balb3T3 cell is probed using two different-sized particles, and the localized cell stiffness is evaluated using Hertz model. A series of experiments are performed to obtain the necessary parameters for the cell stiffness computation: particle displacement, trapping stiffness, force exertion, and cell deformation. The computed cell stiffness measurements are 17 and 40 Pa using 4 μm- and 2 μm-sized particles, respectively. Results suggest that the proposed optical tweezers scheme can measure the stiffness of a particular cell locale using Hertz model, offering insights about how cells respond to outside mechanical stimulus.
提出了一种简单的光镊设计，用于在轴向方向上操纵粒子，并以纳米灵敏度估计粒子位置。使用两种不同尺寸的粒子探测 Balb3T3 细胞，并使用赫兹模型评估局部细胞刚度。进行了一系列实验以获得细胞刚度计算所需的参数：粒子位移、捕获刚度、力施加和细胞变形。使用 4μm 和 2μm 大小的粒子分别获得了 17 和 40Pa 的计算细胞刚度测量值。结果表明，所提出的光镊方案可以使用赫兹模型测量特定细胞位置的刚度，提供有关细胞如何对外界机械刺激做出反应的见解。
