Chernikov Anatoly V, Bruskov Vadim I, Gudkov Sergey V
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3 Institutskaya St., Pushchino, Moscow Region, 142290, Russia.
J Biol Phys. 2013 Sep;39(4):687-99. doi: 10.1007/s10867-013-9330-z. Epub 2013 Aug 10.
It was found by the fluorimetric method using 2,3-diaminonaphthalene that moderate heating of water (60-80°C, for up to 4 h) leads to the fixation of atmospheric nitrogen with the formation of nitrite. The kinetic parameters of this process were determined. The energy of activation of [Formula: see text]formation was estimated to be 139 kJ/mol. It was found that the amount of nitrite formed depends on the concentration of dissolved oxygen and nitrogen. It was shown by two independent methods (Griess reagent/VCl3 and 2,3-diaminonaphthalene/nitrate reductase) that heating of water (80°C, 1 h) results in the formation of nitrate; with the use of the fluorescent probe dihydrorhodamine 123, the generation of nitrogen dioxide (peroxynitrite) was revealed. Nitrite, nitrate, and nitrogen dioxide are formed in water upon heating in approximately equal amounts. A scheme of reactions proceeding with bidistilled water by the action of heat with the formation of nitrogen oxides is proposed.
采用2,3 - 二氨基萘荧光法发现，对水进行适度加热（60 - 80°C，长达4小时）会导致大气氮固定并形成亚硝酸盐。测定了该过程的动力学参数。[公式：见原文]形成的活化能估计为139 kJ/mol。发现形成的亚硝酸盐量取决于溶解氧和氮的浓度。通过两种独立方法（格里斯试剂/VCl₃和2,3 - 二氨基萘/硝酸还原酶）表明，水加热（80°C，1小时）会导致硝酸盐形成；使用荧光探针二氢罗丹明123，揭示了二氧化氮（过氧亚硝酸根）的生成。加热时，水中形成的亚硝酸盐、硝酸盐和二氧化氮的量大致相等。提出了通过热作用在双蒸水中进行反应并形成氮氧化物的反应方案。
