Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110, Safat, Kuwait,
Obes Surg. 2014 Jan;24(1):95-101. doi: 10.1007/s11695-013-1056-2.
Currently, the most effective treatment for obesity is bariatric surgery. Gastroduodenal bypass surgery produces sustained weight loss and improves glycemic control and insulin sensitivity. Previous studies have shown that sleeve gastrectomy (SG) produces similar results and implicate changes in incretin hormone release in these effects.
Male Sprague-Dawley rats were divided into four groups; lean control (lean), diet-induced obesity (DIO), DIO animals that had undergone SG (SG), and DIO animals that had undergone a sham operation (sham).
After a 2-week recovery period, the incretin response to a standard test meal was measured. Blood sampling was performed in free-moving rats at various time points using chronic vascular access to the right jugular vein. There was a significant increase in the bodyweight of DIO animals fed a high-fat/high-sugar diet compared with the lean animals, which was reversed by SG. DIO caused an impairment of the GLP-1 response to a standard test meal, but not the GIP response. SG resulted in a dramatic increase in the GLP-1 response to a standard test meal but had no effect on the GIP response.
A rapid rise in blood sugar was observed in the SG group following a standard test meal that was followed by reactive hypoglycemia. SG dramatically increases the GLP-1 response to a standard test meal but has no effect on GIP in a rat model of DIO.
目前，治疗肥胖症最有效的方法是减重手术。胃旁路手术可产生持续的体重减轻，并改善血糖控制和胰岛素敏感性。先前的研究表明，袖状胃切除术（SG）可产生相似的效果，并暗示在这些作用中肠促胰岛素激素释放的变化。
雄性 Sprague-Dawley 大鼠分为四组：瘦对照组（lean）、饮食诱导肥胖组（DIO）、接受 SG 的 DIO 动物组（SG）和接受假手术的 DIO 动物组（sham）。
在 2 周的恢复期后，测量了标准餐试验的肠促胰岛素反应。通过对右侧颈静脉的慢性血管通路，在自由移动的大鼠中在不同时间点进行采血。与 lean 动物相比，高脂肪/高糖饮食喂养的 DIO 动物体重显著增加，SG 可逆转这一情况。DIO 导致标准餐试验中 GLP-1 反应受损，但 GIP 反应不受影响。SG 导致标准餐试验中 GLP-1 反应明显增加，但对 GIP 反应没有影响。
在标准餐试验后，SG 组的血糖迅速升高，随后出现反应性低血糖。SG 可显著增加标准餐试验中 GLP-1 的反应，但对 DIO 大鼠模型中的 GIP 没有影响。
