Kuang Rong-Guang, Wu Heng-Xiang, Hao Guo-Xiang, Wang Jian-Wen, Zhou Cheng-Jun
The Second Hospital of Shandong University, Department of Pathology, Jinan, China.
Turk J Gastroenterol. 2013;24(2):99-108. doi: 10.4318/tjg.2013.0571.
BACKGROUND/AIMS: By detecting the expression and distribution of insulin-like growth factor-2, proliferating cell nuclear antigen, and matrix metalloproteinase-7 in cancer cells and the expression of α-actin in interstitial myofibroblasts, we studied their differences and their relationship in intestinal type and diffuse type gastric cancer with Lauren classification.
Clinical and pathological data of 50 patients with gastric adenocarcinoma who underwent primary surgical resection between 2003 and 2008 in Qianfoshan Hospital were collected. The cancer was classified as intestinal or diffuse type with Lauren classification. Immunohistochemical technique was used to detect the protein expression of insulin-like growth factor-2, proliferating cell nuclear antigen, matrix metalloproteinase-7, and α-actin in both gastric cancer tissue and normal gastric mucosa.
The expression of insulin-like growth factor-2, proliferating cell nuclear antigen, matrix metalloproteinase-7, and α-actin in the gastric tissue was significantly higher than in the normal gastric mucosa. Insulin-like growth factor-2 was mainly expressed in the nucleus in diffuse gastric cancer and in the cytoplasm in intestinal type. The expression of insulin-like growth factor-2 in the nucleus was correlated with depth of tumor invasion, lymph node metastasis and staging. Proliferating cell nuclear antigen and matrix metalloproteinase-7 showed higher expression in diffuse than in intestinal type gastric cancer. The overexpression of proliferating cell nuclear antigen was relevant to lymph node metastasis in gastric cancer. The overexpression of matrix metalloproteinase-7 was relevant to invasion, lymph node metastasis, distant metastasis, and staging in gastric cancer. There was no significant difference in α-actin expression between the intestinal type and diffuse type gastric cancer. The overexpression of α-actin was relevant to cancer invasion and lymph node metastasis.
The expression patterns of insulin-like growth factor-2 and the expression intensity of proliferating cell nuclear antigen and matrix metalloproteinase-7 were significantly different between diffuse type and intestinal type gastric cancer cells, but the expression pattern and intensity of the interstitial myofibroblast marker showed no significant difference. The clinical pathology distinction between intestinal type and diffuse type gastric cancer may be due mainly to the change in the genetic structure and the phenotype of epithelial cells, and interstitial myofibroblasts and cancer cells jointly promote the invasion and metastasis of gastric cancer.
背景/目的：通过检测胰岛素样生长因子-2、增殖细胞核抗原和基质金属蛋白酶-7在癌细胞中的表达及分布，以及α-肌动蛋白在间质肌成纤维细胞中的表达，我们采用劳伦分类法研究了它们在肠型和弥漫型胃癌中的差异及其关系。
收集2003年至2008年在千佛山医院接受初次手术切除的50例胃腺癌患者的临床和病理资料。根据劳伦分类法将癌症分为肠型或弥漫型。采用免疫组织化学技术检测胰岛素样生长因子-2、增殖细胞核抗原、基质金属蛋白酶-7和α-肌动蛋白在胃癌组织和正常胃黏膜中的蛋白表达。
胃组织中胰岛素样生长因子-2、增殖细胞核抗原、基质金属蛋白酶-7和α-肌动蛋白的表达明显高于正常胃黏膜。胰岛素样生长因子-2在弥漫型胃癌中主要表达于细胞核，在肠型中表达于细胞质。细胞核中胰岛素样生长因子-2的表达与肿瘤浸润深度、淋巴结转移及分期相关。增殖细胞核抗原和基质金属蛋白酶-7在弥漫型胃癌中的表达高于肠型。增殖细胞核抗原的过表达与胃癌的淋巴结转移相关。基质金属蛋白酶-7的过表达与胃癌的浸润、淋巴结转移、远处转移及分期相关。肠型和弥漫型胃癌中α-肌动蛋白的表达无显著差异。α-肌动蛋白的过表达与癌症浸润和淋巴结转移相关。
弥漫型和肠型胃癌细胞中胰岛素样生长因子-2的表达模式以及增殖细胞核抗原和基质金属蛋白酶-7的表达强度存在显著差异，但间质肌成纤维细胞标志物的表达模式和强度无显著差异。肠型和弥漫型胃癌的临床病理差异可能主要归因于上皮细胞基因结构和表型的改变，间质肌成纤维细胞和癌细胞共同促进胃癌的侵袭和转移。
