Martín-Núñez Gracia María, Cabrera-Mulero Rebeca, Rojo-Martínez Gemma, Gómez-Zumaquero Juan Miguel, Chaves Felipe Javier, de Marco Griselda, Soriguer Federico, Castaño Luis, Morcillo Sonsoles
Endocrinology and Nutrition Service, Hospital Carlos Haya, Instituto de Investigación Biomédica (IBIMA), Malaga, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) of the Instituto de Salud Carlos III, Barcelona, Spain.
Mol Nutr Food Res. 2013 Dec;57(12):2177-84. doi: 10.1002/mnfr.201300208. Epub 2013 Aug 12.
The serum fatty acid (FA) composition is influenced by dietary fat and the endogenous production of FAs. Stearoyl CoA desaturase 1 (SCD1) is the rate-limiting enzyme catalyzing the synthesis of MUFAs from saturated FAs. Variations in SCD1 activity have been associated with obesity, diabetes, or inflammation. We evaluated the associations between genetic variation of the SCD1 gene, SCD1 activity, intake of oil, and obesity in a population-based prospective study in southern Spain.
We collected phenotypic, metabolic, nutritional, and genetic information. The type of dietary fat was assessed from samples of cooking oil taken from the participants' kitchens and analyzed by GC. A total of nine single nucleotide polymorphisms (SNPs) of the SCD1 gene were analyzed by SNPlex technology. We found a significant association between SCD1 genetic variation and enzyme activity in four of nine polymorphisms studied. An interaction between rs10883463 and olive oil intake on the [18:1/18:0] desaturase index was found (p = 0.009). We also showed that genetic variations in the SCD1 gene were associated with obesity.
Our results show a relationship between genetic variation of the SCD1 gene, enzyme activity, and the risk of obesity, an association that is not independent of the type of oil consumed.
血清脂肪酸（FA）组成受膳食脂肪和脂肪酸内源性生成的影响。硬脂酰辅酶A去饱和酶1（SCD1）是催化从饱和脂肪酸合成单不饱和脂肪酸的限速酶。SCD1活性的变化与肥胖、糖尿病或炎症有关。在西班牙南部一项基于人群的前瞻性研究中，我们评估了SCD1基因的遗传变异、SCD1活性、食用油摄入量与肥胖之间的关联。
我们收集了表型、代谢、营养和遗传信息。膳食脂肪类型通过从参与者厨房采集的食用油样本进行评估，并通过气相色谱法进行分析。通过SNPlex技术分析了SCD1基因的总共9个单核苷酸多态性（SNP）。我们在所研究的9个多态性中的4个中发现SCD1遗传变异与酶活性之间存在显著关联。发现rs10883463与橄榄油摄入量对[18:1/18:0]去饱和酶指数有相互作用（p = 0.009）。我们还表明SCD1基因的遗传变异与肥胖有关。
我们的结果表明SCD1基因的遗传变异、酶活性与肥胖风险之间存在关联，这种关联并非独立于所消费的油的类型。
