用抗体对纳米颗粒进行功能化修饰的技巧。

Tips for the functionalization of nanoparticles with antibodies.

作者信息

Polo Ester, Puertas Sara, Moros María, Batalla Pilar, Guisán José M, de la Fuente Jesús M, Grazú Valeria

机构信息

Nanotherapy and Nanodiagnostics Group (GN2), Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Zaragoza, Spain.

出版信息

Methods Mol Biol. 2013;1051:149-63. doi: 10.1007/978-1-62703-550-7_11.

DOI:10.1007/978-1-62703-550-7_11

PMID:23934804

Abstract

Multiple antibody immobilization methodologies have been developed for several applications including affinity chromatography, immunosensing, and drug delivery. Most of them have been carried out without considering the orientation of the antigen binding site of the antibody, or after the chemical modification of the antibody. An efficient immobilization to improve the biological activity of the antibody is one of the key fundamental issues to pursue. A simple and effective methodology for well-oriented covalently immobilization of antibodies on nanoparticles is reported in this chapter.

摘要

已经开发了多种抗体固定方法用于多种应用，包括亲和色谱、免疫传感和药物递送。其中大多数方法在进行时没有考虑抗体抗原结合位点的方向，或者是在抗体化学修饰之后进行的。提高抗体生物活性的有效固定是需要解决的关键基本问题之一。本章报道了一种简单有效的将抗体定向共价固定在纳米颗粒上的方法。