Strack Guinevere, Nichols Robert, Atanassov Plamen, Luckarift Heather R, Johnson Glenn R
Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
Methods Mol Biol. 2013;1051:217-28. doi: 10.1007/978-1-62703-550-7_14.
Conductive materials functionalized with redox enzymes provide bioelectronic architectures with application to biological fuel cells and biosensors. Effective electron transfer between the enzyme (biocatalyst) and the conductive materials is imperative for function. Various nanostructured carbon materials are common electrode choices for these applications as both the materials' inherent conductivity and physical integrity aids optimal performance. The following chapter presents a method for the use of carbon nanotube buckypaper as a conductive architecture suitable for biocatalyst functionalization. In order to securely attach the biocatalyst to the carbon nanotube surface, the conductive buckypaper is modified with the heterobifunctional cross-linker, 1-pyrenebutanoic acid, succinimidyl ester. The technique effectively tethers the enzyme to the carbon nanotube which enhances bioelectrocatalysis, preserves the conductive nature of the carbon surface, and facilities direct electron transfer between the catalyst and material interface. The approach is demonstrated using phenol oxidase (laccase) and pyrroloquinoline quinone-dependent glucose dehydrogenase PQQ-GDH, as representative biocatalysts.
用氧化还原酶功能化的导电材料为生物燃料电池和生物传感器提供了生物电子架构。酶（生物催化剂）与导电材料之间的有效电子转移对于其功能至关重要。各种纳米结构的碳材料是这些应用中常见的电极选择，因为材料的固有导电性和物理完整性有助于实现最佳性能。以下章节介绍了一种使用碳纳米管巴基纸作为适合生物催化剂功能化的导电架构的方法。为了将生物催化剂牢固地附着在碳纳米管表面，用异双功能交联剂1-芘丁酸琥珀酰亚胺酯对导电巴基纸进行修饰。该技术有效地将酶束缚在碳纳米管上，增强了生物电催化作用，保持了碳表面的导电性质，并促进了催化剂与材料界面之间的直接电子转移。使用酚氧化酶（漆酶）和吡咯喹啉醌依赖性葡萄糖脱氢酶PQQ-GDH作为代表性生物催化剂证明了该方法。
