Sánchez Pello, Hernández Rosa María, Pedraz José Luis, Orive Gorka
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country, Vitoria-Gasteiz, Spain.
Methods Mol Biol. 2013;1051:313-25. doi: 10.1007/978-1-62703-550-7_21.
Cell microencapsulation is based on the immobilization of cells for continuous release of therapeutics. This approach has been tested in the treatment of many diseases and several clinical trials have been performed. Factors such as the choice of cells to be encapsulated, the biomaterial used, and the procedure for carrying out the capsules are important issues when implementing this technology.This book chapter makes a comprehensive description of alginate, the most frequently employed biomaterial, passing by its structure, the extraction and treatment, and finishing with the process of gelation. It also describes the various modifications that can be carried out to allow the interaction between the alginate and the integrin receptors of encapsulated cells. The main microencapsulation technologies are presented as well as how 100 μm alginate-Poly-L-Lysine-alginate microcapsules can be fabricated with Flow-focusing technology.
细胞微囊化基于细胞固定化以实现治疗剂的持续释放。这种方法已在多种疾病的治疗中进行了测试，并开展了多项临床试验。在实施这项技术时，诸如待封装细胞的选择、所用生物材料以及制备微囊的程序等因素都是重要问题。本章对最常用的生物材料藻酸盐进行了全面描述，涵盖其结构、提取与处理，最后介绍了凝胶化过程。还描述了为使藻酸盐与封装细胞的整合素受体相互作用而可进行的各种修饰。介绍了主要的微囊化技术以及如何利用流动聚焦技术制备100μm的藻酸盐-聚-L-赖氨酸-藻酸盐微囊。
