Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany; Department of Physics, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
Int J Biol Macromol. 2015;78:72-8. doi: 10.1016/j.ijbiomac.2015.03.061. Epub 2015 Apr 4.
Hydrogel-based biomaterials are ideal scaffolding matrices for microencapsulation, but they need to be modified to resemble the mechanical, structural and chemical properties of the native extracellular matrix. Here, we compare the mechanical properties and the degradation behavior of unmodified and modified alginate hydrogels in which cell adhesive functionality is conferred either by blending or covalently cross-linking with gelatin. Furthermore, we measure the spreading and proliferation of encapsulated osteoblast-like MG-63 cells. Alginate hydrogels covalently crosslinked with gelatin show the highest degree of cell adhesion, spreading, migration, and proliferation, as well as a faster degradation rate, and are therefore a particularly suitable material for microencapsulation.
水凝胶基生物材料是微胶囊化的理想支架基质，但需要进行修饰以模拟天然细胞外基质的机械、结构和化学性质。在这里，我们比较了未修饰和修饰的藻酸盐水凝胶的机械性能和降解行为，其中细胞黏附功能是通过与明胶共混或共价交联赋予的。此外，我们还测量了封装的成骨样 MG-63 细胞的扩展和增殖。用明胶共价交联的藻酸盐水凝胶表现出最高的细胞黏附、扩展、迁移和增殖程度，以及更快的降解速度，因此是微胶囊化的特别合适的材料。
