Moreno-Garrido Ignacio
Department of Ecology and Coastal Management, Institute of Marine Sciences of Andalucia (ICMAN-CSIC), Cádiz, Spain.
Methods Mol Biol. 2013;1051:327-47. doi: 10.1007/978-1-62703-550-7_22.
In this review, methods for the most common microalgal immobilization procedures are gathered and described. Passive (due to natural adherence of cells to surfaces) and active immobilization methods should be distinguished. Among active immobilization methods, calcium alginate entrapment is the most widely used method if living cells are intended to be immobilized, due to the chemical, optical, and mechanical characteristics of this substance. Immobilization in synthetic foams, immobilization in agar and carrageenan as well as immobilization in silica-based matrix or filters are also discussed and described. Finally, some considerations on the use of flocculation for microalgae are mentioned.
在本综述中，收集并描述了最常见的微藻固定化程序的方法。应区分被动（由于细胞自然附着于表面）和主动固定化方法。在主动固定化方法中，如果要固定活细胞，由于藻酸钙的化学、光学和机械特性，藻酸钙包埋是最广泛使用的方法。还讨论并描述了在合成泡沫中固定化、在琼脂和角叉菜胶中固定化以及在基于二氧化硅的基质或过滤器中固定化。最后，提到了一些关于微藻絮凝使用的注意事项。
