Perullini Mercedes, Ferro Yannis, Durrieu Claude, Jobbágy Matías, Bilmes Sara A
INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina.
Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés, UMR 5023 ENTPE, Université de Lyon, France.
J Biotechnol. 2014 Jun 10;179:65-70. doi: 10.1016/j.jbiotec.2014.02.007. Epub 2014 Mar 15.
An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods.
基于TAFR（四乙氧基硅烷无醇路线合成）在二氧化硅基质中对微藻进行酸性封装，开发了一种具有改进光学质量的先进混合生物传感平台。此前，按照两步法将三种微藻（普通小球藻、斜生栅藻和莱茵衣藻）固定在藻酸盐中。借助Tris-HCl缓冲液调节藻酸盐的保护功能，在远低于被封装微藻耐受极限的pH 4.0下进行溶胶-凝胶合成。Si(IV)的酸性缩合生成了具有出色光学特性的二氧化硅基质，符合基于光学检测方法的生物传感器的要求。
