Azari Hassan
Department of Anatomical Sciences, Neural Stem Cell and Regenerative Neuroscience Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran.
Methods Mol Biol. 2013;1059:95-106. doi: 10.1007/978-1-62703-574-3_9.
The renewable source of neural stem cells (NSCs) with multi-lineage differentiation capability towards neurons, astrocytes, and oligodendrocytes represent an ideal supply for cell therapy of central nervous system (CNS) diseases. In spite of this, the clinical use of NSCs is hampered by heterogeneity, poor neuronal cell yield, predominant astrocytic differentiation of NSC progeny and possible uncontrolled proliferation, and tumor formation upon transplantation. The ability to generate highly enriched and defined neural cell populations from the renewable source of NSCs might overcome many of these impediments and pave the way towards their successful clinical applications. Here, we describe a simple method for NSC differentiation and subsequent purification of neuronal progenitor cells, taking advantage of size and granularity differences between neuronal cells and other NSC progeny. This highly enriched neuronal cell population provides an invaluable source of cells for both in vitro and in vivo studies.
具有向神经元、星形胶质细胞和少突胶质细胞多谱系分化能力的神经干细胞（NSCs）的可再生来源，是中枢神经系统（CNS）疾病细胞治疗的理想细胞来源。尽管如此，神经干细胞的临床应用受到其异质性、神经元细胞产量低、神经干细胞后代主要向星形胶质细胞分化以及可能的不受控制的增殖和移植后肿瘤形成的阻碍。从可再生的神经干细胞来源生成高度富集且明确的神经细胞群体的能力，可能会克服其中许多障碍，并为其成功的临床应用铺平道路。在此，我们描述了一种利用神经元细胞与其他神经干细胞后代之间的大小和颗粒度差异，对神经干细胞进行分化以及随后纯化神经元祖细胞的简单方法。这种高度富集的神经元细胞群体为体外和体内研究提供了宝贵的细胞来源。
