Azari Hassan, Reynolds Brent A
Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611 Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences & Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611.
Cold Spring Harb Perspect Biol. 2016 Jun 1;8(6):a021279. doi: 10.1101/cshperspect.a021279.
The process of generating new neurons of different phenotype and function from undifferentiated stem and progenitor cells starts at very early stages of development and continues in discrete regions of the mammalian nervous system throughout life. Understanding mechanisms underlying neuronal cell development, biology, function, and interaction with other cells, especially in the neurogenic niche of fully developed adults, is important in defining and developing new therapeutic regimes in regenerative neuroscience. Studying these complex and dynamic processes in vivo is challenging because of the complexity of the nervous system and the presence of many known and unknown confounding variables. However, the challenges could be overcome with simple and robust in vitro models that more or less recapitulate the in vivo events. In this work, we will present an overview of present available in vitro cell-based models of neurogenesis.
从未分化的干细胞和祖细胞生成具有不同表型和功能的新神经元的过程始于发育的非常早期阶段，并在整个生命过程中在哺乳动物神经系统的离散区域持续进行。了解神经元细胞发育、生物学、功能以及与其他细胞相互作用的潜在机制，特别是在完全发育的成年人的神经发生微环境中，对于定义和开发再生神经科学中的新治疗方案至关重要。由于神经系统的复杂性以及存在许多已知和未知的混杂变量，在体内研究这些复杂和动态的过程具有挑战性。然而，使用或多或少能重现体内事件的简单而强大的体外模型可以克服这些挑战。在这项工作中，我们将概述目前可用的基于细胞的神经发生体外模型。
