从人嗅黏膜中分离成体干细胞。

Isolation of adult stem cells from the human olfactory mucosa.

作者信息

Féron François, Perry Chris, Girard Stéphane D, Mackay-Sim Alan

机构信息

Aix Marseille University, NICN, CNRS UMR 7259,Center for Clinical Investigations in Biotherapy, Marseille, France.

出版信息

Methods Mol Biol. 2013;1059:107-14. doi: 10.1007/978-1-62703-574-3_10.

DOI:10.1007/978-1-62703-574-3_10

PMID:23934838

Abstract

The olfactory mucosa, located in the nasal cavity, is the only nervous tissue that is exposed to the external environment and easily accessible in every living individual. In addition, this organ is home of a continuing neurogenesis that is sustained by a large population of stem cells. Here, we describe a method for biopsying olfactory mucosa from human nasal cavities and isolating multipotent adult stem cells that can be used to either identify biomarkers in brain disorders or repair the pathological/traumatized nervous system.

摘要

位于鼻腔内的嗅黏膜是唯一暴露于外部环境且每个个体都易于获取的神经组织。此外，该器官是持续神经发生的场所，由大量干细胞维持。在此，我们描述一种从人鼻腔获取嗅黏膜活检样本并分离多能成体干细胞的方法，这些干细胞可用于识别脑部疾病中的生物标志物或修复病理/创伤性神经系统。