Louis Sharon A, Mak Carmen K H
STEMCELL Technologies Inc., Vancouver, Canada.
Methods Mol Biol. 2013;1059:117-32. doi: 10.1007/978-1-62703-574-3_11.
Recent reports have highlighted several parameters of the neurosphere culture or assay system which render it unreliable as a quantitative in vitro assay for measuring neural stem cell (NSC) frequency. The single-step semi-solid based assay, the Neural Colony Forming Cell (NCFC) assay is an assay which was developed to overcome some of the limitations of the neurospheres assay in terms of accurately measuring NSC numbers. The NCFC assay allows the discrimination between NSCs and progenitors by the size of colonies they produce (i.e. their proliferative potential). The NCFC assay and other improved tissue culture tools offer further advances in the promising application of NSCs for therapeutic use.
最近的报告强调了神经球培养或检测系统的几个参数，这些参数使其作为测量神经干细胞（NSC）频率的定量体外检测方法不可靠。基于单步半固体的检测方法，即神经集落形成细胞（NCFC）检测，是为克服神经球检测在准确测量NSC数量方面的一些局限性而开发的检测方法。NCFC检测通过NSC产生的集落大小（即其增殖潜力）来区分NSC和祖细胞。NCFC检测和其他改进的组织培养工具为NSC在治疗应用中的前景提供了进一步的进展。
