Cavazzin Chiara, Neri Margherita, Gritti Angela
Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute, San Raffaele Telethon Institute for Gene Therapy, Milan, Italy.
Methods Mol Biol. 2013;1059:25-40. doi: 10.1007/978-1-62703-574-3_3.
Due to the complexity of the NSC niche organization, the lack of specific NSC markers and the difficulty of long-term tracking these cells and their progeny in vivo the functional properties of the endogenous NSCs remain largely unexplored. These limitations have led to the development of methodologies to efficiently isolate, expand, and differentiate NSCs ex vivo. We describe here the peculiarities of the neurosphere assay (NSA) as a methodology that allows to efficiently isolate, expand, and differentiate somatic NSCs derived from the adult forebrain periventricular region while preserving proliferation, self-renewal, and multipotency, the main attributes that provide their functional identification.
由于神经干细胞生态位组织的复杂性、缺乏特异性神经干细胞标志物以及在体内长期追踪这些细胞及其后代的困难，内源性神经干细胞的功能特性在很大程度上仍未得到探索。这些局限性促使了体外高效分离、扩增和分化神经干细胞方法的发展。我们在此描述神经球测定法（NSA）的特点，它作为一种方法能够有效地分离、扩增和分化源自成年前脑脑室周围区域的体细胞神经干细胞，同时保留增殖、自我更新和多能性这些赋予其功能识别的主要特性。
