Chen Xiao-Yu, Wang Zhi-Cheng, Li Jun, Liu Xiao-Li, Sun Yu-Hua
Department of Histology and Embryology, Anhui Medical University, Hefei, Anhui 230032;
Exp Ther Med. 2013 Jul;6(1):172-176. doi: 10.3892/etm.2013.1078. Epub 2013 Apr 25.
The aim of this study was to investigate the preventive effects of resveratrol (Res) on rats with adjuvant arthritis (AA) and the mechanism(s) of action. An AA model was established by injection of Freund's complete adjuvant (FCA). Vascular endothelial growth factor (VEGF) was visualized in joint specimens using immunohistochemistry. IL-1β and TNF-α production in synoviocytes was determined by radioimmunoassay (RIA). The mRNA expression of IL-1β and TNF-α was observed in synoviocytes using the reverse transcription (RT)-PCR method. The synoviocytes of the AA model were stimulated by Res or treated with the protein kinase C (PKC) inhibitor chelerythrine prior to stimulation. The expression of phosphorylated ERK1/2 (p-ERK1/2) was detected by western blotting. Res was able to reduce the elevated levels of IL-1β and TNF-α, and inhibit the mRNA expression of IL-1β and TNF-α in the synoviocytes of the AA model rats. VEGF expression in the Res-treated group was significantly lowered. The protein expression levels of p-ERK1/2 were significantly higher in the Res-treated group compared with those of the model group, while p-ERK1/2 was markedly lower in the group pretreated with chelerythrine. Res has a therapeutic effect on AA rats, which may be correlated with its immunoregulatory actions, and may activate p-ERK1/2 in synoviocytes via PKC.
本研究旨在探讨白藜芦醇（Res）对佐剂性关节炎（AA）大鼠的预防作用及其作用机制。通过注射弗氏完全佐剂（FCA）建立AA模型。采用免疫组织化学法观察关节标本中血管内皮生长因子（VEGF）的表达。采用放射免疫分析法（RIA）检测滑膜细胞中IL-1β和TNF-α的产生。采用逆转录（RT）-PCR法观察滑膜细胞中IL-1β和TNF-α的mRNA表达。用Res刺激AA模型的滑膜细胞，或在刺激前用蛋白激酶C（PKC）抑制剂白屈菜红碱处理。采用蛋白质印迹法检测磷酸化ERK1/2（p-ERK1/2）的表达。Res能够降低AA模型大鼠滑膜细胞中升高的IL-1β和TNF-α水平，并抑制IL-1β和TNF-α的mRNA表达。Res治疗组的VEGF表达明显降低。与模型组相比，Res治疗组p-ERK1/2的蛋白表达水平明显升高，而白屈菜红碱预处理组p-ERK1/2明显降低。Res对AA大鼠有治疗作用，这可能与其免疫调节作用有关，并且可能通过PKC激活滑膜细胞中的p-ERK1/2。
