Zagursky R J, McCormick R M
Central Research and Development Department, E.I. du Pont de Nemours and Company, Wilmington, DE 19880-0328.
Biotechniques. 1990 Jul;9(1):74-9.
DNA sequencing separations of standard DNA fragments of known sequence have been achieved in small diameter capillary gels electrophoresed and analyzed in parallel in a modified commercial DNA sequencer instrument. DNA sequencing in terms of base-calling accuracy is comparable to conventional slab gels; however, the separations in the capillary were performed somewhat faster and required less sample than those in the slab gel. Advantages of this approach vs. separations on conventional slab gels are discussed.
在一台经过改装的商用DNA测序仪中，对已知序列的标准DNA片段进行了小直径毛细管凝胶电泳分离，并并行分析。就碱基识别准确性而言，DNA测序结果与传统平板凝胶相当；然而，毛细管中的分离速度更快，所需样品比平板凝胶中的少。本文讨论了这种方法相对于传统平板凝胶分离的优势。
