Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, China.
PLoS One. 2013 Jul 30;8(7):e69141. doi: 10.1371/journal.pone.0069141. Print 2013.
Paeoniflorin (PF), which is the main active ingredient in the root of Paeonia Radix, has many pharmacological effects. Here, we investigated the effect of PF on rat pulmonary artery smooth muscle cells (PASMCs) under hypoxic conditions and explored the mechanisms of the effects. The anti-proliferative effect of PF increased in a dose dependent manner. At the highest dose (20 μmol/L), the anti-proliferative effect of PF peaked at 24 h after administration. However, the selective A2B adenosine receptor (A2BAR) antagonist MRS1754 abolished it. PF increased A2BAR mRNA levels from 0.0763±0.0067 of β-actin mRNA levels (hypoxia group) to 0.1190±0.0139 (P<0.05) measured by Real Time Reverse Transcription-Polymerase Chain Reaction. A2BAR protein expression measured by Western Blot was also increased. PF inhibited the proliferation of PASMCs by blocking cell cycle progression in the S phase. These data indicated that activation of A2BAR might be involved in the anti-proliferative effect of PF on PASMCs under hypoxic conditions. The results suggested that a new mechanism of PF could be relevant to the management of clinical hypoxic pulmonary hypertension.
芍药苷（PF）是白芍根的主要活性成分，具有多种药理作用。在这里，我们研究了 PF 在低氧条件下对大鼠肺动脉平滑肌细胞（PASMCs）的作用，并探讨了其作用机制。PF 的抗增殖作用呈剂量依赖性增加。在最高剂量（20 μmol/L）时，PF 的抗增殖作用在给药后 24 小时达到峰值。然而，选择性 A2B 腺苷受体（A2BAR）拮抗剂 MRS1754 使其消失。PF 通过实时逆转录聚合酶链反应测量，将 A2BAR mRNA 水平从 0.0763±0.0067 的β-肌动蛋白 mRNA 水平（低氧组）增加到 0.1190±0.0139（P<0.05）。Western Blot 测量的 A2BAR 蛋白表达也增加了。PF 通过阻断 S 期细胞周期进程抑制 PASMCs 的增殖。这些数据表明，A2BAR 的激活可能参与了 PF 在低氧条件下对 PASMCs 的抗增殖作用。结果表明，PF 的一种新机制可能与临床低氧性肺动脉高压的治疗有关。
