Sinha Mau, Singh Amar, Shokeen Akshita, Sharma Pradeep, Kaushik Sanket, Mitra Dipendra K, Kaur Punit, Sharma Sujata, Singh Tej P
Department of Biophysics, All India Institute of Medical Sciences New Delhi-110029, India.
Int J Biochem Mol Biol. 2013 Jul 29;4(2):95-101. Print 2013.
Several plant-derived allergens have been identified which result in the formation of immunoglobulin E antibodies. Primarily, these allergens belong to the protein families including seed storage proteins, structural proteins and pathogenesis-related proteins. Several allergens are also reported from flower bulbs which cause contact dermatitis. Such symptoms are highly common with the bulb growers handling different species of Narcissus. Narcissus toxicity is also reported if the bulbs are consumed accidentally. The present study aimed to characterize the protein from the bulbs of Narcissus tazetta responsible for its allergenic response. A 13 kDa novel allergenic protein, Narcin was isolated from the bulbs of Narcissus tazetta. The protein was extracted using ammonium sulfate fractionation. The protein was further purified by anion exchange chromatography followed by gel filtration chromatography. The N-terminal sequence of the first 15 amino-acid residues was determined using Edman degradation. The allergenicity of the protein was measured by cytokine production using flow cytometry in peripheral blood mononuclear cells. Further estimation of total IgE was performed by ELISA method. This novel protein was found to induce pro-inflammatory cytokines and thus induce allergy by elevating total IgE level. The novel protein, Narcin isolated from Narcissus tazetta was found to exhibit allergenic properties.
已经鉴定出几种植物源性过敏原，它们会导致免疫球蛋白E抗体的形成。这些过敏原主要属于蛋白质家族，包括种子贮藏蛋白、结构蛋白和病程相关蛋白。也有报道称，来自鳞茎花卉的几种过敏原会引起接触性皮炎。这种症状在处理不同种类水仙的鳞茎种植者中非常常见。如果不小心食用了水仙鳞茎，也会有中毒报告。本研究旨在鉴定导致其过敏反应的水仙鳞茎中的蛋白质。从水仙的鳞茎中分离出一种13 kDa的新型过敏原蛋白，水仙素。该蛋白采用硫酸铵分级分离法提取。通过阴离子交换色谱法，然后进行凝胶过滤色谱法进一步纯化该蛋白。使用埃德曼降解法测定前15个氨基酸残基的N端序列。通过流式细胞术检测外周血单核细胞中细胞因子的产生来测量该蛋白的致敏性。通过ELISA法进一步测定总IgE。发现这种新型蛋白可诱导促炎细胞因子，从而通过提高总IgE水平诱导过敏。从水仙中分离出的新型蛋白水仙素被发现具有致敏特性。
