Warren J R, Shaw B, Steinkampf M P
Department of Obstetrics and Gynecology, University of Alabama, Birmingham 35294.
Biol Reprod. 1990 Jul;43(1):158-61. doi: 10.1095/biolreprod43.1.158.
Preimplantation mouse embryos were exposed to nitrous oxide for 30 min to determine its effects on subsequent development after short durations of exposure. Two-cell mouse embryos were exposed to 60% nitrous oxide/40% oxygen at 6-7 h, 3-4 h, or 0-1 h prior to the expected onset of their first cleavage in vitro, or at the 4-cell or morula stages. Effects of nitrous oxide were not observed except in 2-cell embryos treated within 4 h of the expected in vitro cleavage. At 3-4 h and 0-1 h prior to the onset of cleavage, exposure to 60% nitrous oxide/40% oxygen resulted in blastocyst development rates of 27.7% and 4.7%, respectively, while control rates ranged from 75% to 77%. The majority of affected embryos were halted at the 2-cell stage before completing cell division. Similar effects were obtained with 80% nitrous oxide/20% oxygen. Thus, we conclude that brief exposure of mouse preimplantation embryos to nitrous oxide may be deleterious to subsequent embryo cleavage, but this effect is highly dependent on the developmental stage at which exposure occurs.
将植入前的小鼠胚胎暴露于一氧化二氮中30分钟，以确定短时间暴露后其对后续发育的影响。在体外预期首次卵裂开始前6 - 7小时、3 - 4小时或0 - 1小时，或者在4细胞或桑椹胚阶段，将二细胞小鼠胚胎暴露于60%一氧化二氮/40%氧气中。除了在预期体外卵裂4小时内处理的二细胞胚胎外，未观察到一氧化二氮的影响。在卵裂开始前3 - 4小时和0 - 1小时，暴露于60%一氧化二氮/40%氧气中，囊胚发育率分别为27.7%和4.7%，而对照率在75%至77%之间。大多数受影响的胚胎在完成细胞分裂前停滞在二细胞阶段。用80%一氧化二氮/20%氧气也获得了类似的结果。因此，我们得出结论，小鼠植入前胚胎短暂暴露于一氧化二氮可能对后续胚胎卵裂有害，但这种影响高度依赖于暴露时的发育阶段。
