Jonderko Krzysztof, Skałba Piotr, Kamińska Magdalena, Kasicka-Jonderko Anna, Galas Ewa, Biały Aleksandra
* Department of Basic Biomedical Science, School of Pharmacy in Sosnowiec.
Eur J Contracept Reprod Health Care. 2013 Oct;18(5):401-9. doi: 10.3109/13625187.2013.819847. Epub 2013 Aug 12.
To examine liver mitochondrial function in women using combined oral contraceptives (COCs) containing ethinylestradiol.
A breath test after oral administration of 1 mg/kg (13)C-alpha-ketoisocaproic acid ((13)C-KICA) and 20 mg/kg L-leucine was performed twice: (i) in 15 women on day 14, 15, 16, 17 or 18 of COC intake, and between day 1 and 5 of the withdrawal bleeding; and (ii) in 15 regularly menstruating females not taking hormonal contraceptives: during the luteal phase, between the 18th and the 22nd day of the cycle, and again between day 1 and 5 of the menstruation.
In women on COCs the maximum (13)C elimination in breath air (Dmax) was higher (26.8 ± 1.6%/h) than during withdrawal bleeding (23.5 ± 1.2%/h; p = 0.012). The time to reach the Dmax was similar on the two study days: 33.3 ± 2.4 min during the phase of pill intake vs. 37.0 ± 2.5 min during the pill-free interval. The one-hour cumulative breath (13)C elimination was greater after two weeks of COC intake than during the withdrawal bleeding: 17.49 ± 1.03% vs. 15.32 ± 0.85% (p = 0.024). In the control group no menstrual cycle phase-dependent fluctuations in the results of the (13)C-KICA breath test were observed.
The metabolism of (13)C-alpha-ketoisocaproic acid augments during the intake of COCs containing ethinylestradiol, reflecting enhanced liver mitochondrial metabolic activity.
研究使用含炔雌醇复方口服避孕药（COC）的女性的肝脏线粒体功能。
口服1mg/kg ¹³C-α-酮异己酸（¹³C-KICA）和20mg/kg L-亮氨酸后进行呼气试验，共进行两次：（i）15名服用COC的女性在服药第14、15、16、17或18天以及撤药性出血第1至5天进行；（ii）15名未服用激素避孕药的规律月经女性：在黄体期，月经周期第18至22天，以及月经第1至5天再次进行。
服用COC的女性呼气中¹³C的最大消除率（Dmax）高于撤药性出血期间（26.8±1.6%/小时对23.5±1.2%/小时；p = 0.012）。达到Dmax的时间在两个研究日相似：服药期为33.3±2.4分钟，停药间期为37.0±2.5分钟。服用COC两周后的一小时累积呼气¹³C消除率高于撤药性出血期间：17.49±1.03%对15.32±0.85%（p = 0.024）。对照组未观察到¹³C-KICA呼气试验结果的月经周期阶段依赖性波动。
服用含炔雌醇的COC期间，¹³C-α-酮异己酸的代谢增强，反映肝脏线粒体代谢活性增强。
