硒生物利用度增加时硒蛋白 M 转基因大鼠肾脏的蛋白质组学分析。

Proteomic analysis of kidneys from selenoprotein M transgenic rats in response to increased bioability of selenium.

机构信息

Department of Biomaterials Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, South Korea.

Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.

出版信息

Clin Proteomics. 2013 Aug 12;10(1):10. doi: 10.1186/1559-0275-10-10.

DOI:10.1186/1559-0275-10-10

PMID:23937859

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3751301/

Abstract

BACKGROUND

To characterize changes in global protein expression in kidneys of transgenic rats overexpressing human selenoprotein M (SelM) in response to increased bioabivility of selenium (Sel), total proteins extracted from kidneys of 10-week-old CMV/hSelM Tg and wild-type rats were separated by 2-dimensional gel electrophoresis and measured for changes in expression.

RESULTS

Ten and three proteins showing high antioxidant enzymatic activity were up- and down-regulated, respectively, in SelM-overexpressing CMV/hSelM Tg rats compared to controls based on an arbitrary 2-fold difference. Up-regulated proteins included LAP3, BAIAP2L1, CRP2, CD73 antigen, PDGF D, KIAA143 homolog, PRPPS-AP2, ZFP313, HSP-60, and N-WASP, whereas down-regulated proteins included ALKDH3, rMCP-3, and STC-1. After Sel treatment, five of the up-regulated proteins were significantly increased in expression in wild-type rats, whereas there were no changes in CMV/hSelM Tg rats. Only two of the down-regulated proteins showed reduced expression in wild-type and Tg rats after Sel treatment.

CONCLUSIONS

These results show the primary novel biological evidences that new functional protein groups and individual proteins in kidneys of Tg rats relate to Sel biology including the response to Sel treatment and SelM expression.

摘要

背景

为了描述在高生物利用度硒（Sel）作用下，过表达人硒蛋白 M（SelM）的转基因大鼠肾脏中全球蛋白质表达的变化，从 10 周龄 CMV/hSelM Tg 和野生型大鼠的肾脏中提取总蛋白，通过二维凝胶电泳分离，并测量其表达变化。

结果

根据 2 倍差异的任意标准，与对照组相比，SelM 过表达的 CMV/hSelM Tg 大鼠中有 10 种和 3 种具有高抗氧化酶活性的蛋白质分别上调和下调。上调的蛋白质包括 LAP3、BAIAP2L1、CRP2、CD73 抗原、PDGF D、KIAA143 同源物、PRPPS-AP2、ZFP313、HSP-60 和 N-WASP，而下调的蛋白质包括 ALKDH3、rMCP-3 和 STC-1。在 Sel 处理后，5 种上调的蛋白质在野生型大鼠中的表达显著增加，而在 CMV/hSelM Tg 大鼠中没有变化。只有 2 种下调的蛋白质在 Sel 处理后在野生型和 Tg 大鼠中表达减少。