Brain Repair Group, School of Bioscience, Cardiff University, Wales, UK.
J Parkinsons Dis. 2012;2(4):309-20. doi: 10.3233/JPD-2012-012133.
Parkinson's disease (PD) is most commonly modelled in rats via injection of the neurotoxin 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle. Assessment of lateralised choice reaction time is usually conducted in the 9 hole box, a highly specialized apparatus that is not widely available in many behavioural laboratories. The retractable lever Skinner box on the other hand is more readily available and may have the additional advantage of faster training of animals.
The present study assesses the performance of lesioned rats on a lateralised choice reaction time task that allows for quantitative assessment of the behavioural profile of models of neurodegenerative diseases in the retractable lever Skinner box.
Here we compared the post lesion performance of pretrained female Lister hooded rats that received unilateral 6-OHDA lesions to the medial forebrain bundle and untreated controls on the choice reaction time task conducted in the Skinner box.
Lesioned animals displayed impairments in contralateral accuracy, a reduced number of usable trials, as well as a slowing down of contralateral reaction and bilateral movement time latencies.
The findings presented allow greater comparison between laboratories, and may be useful for the investigation of treatment strategies and remedies on this model of PD.
帕金森病（PD）最常通过将神经毒素 6-羟多巴胺（6-OHDA）注射到中脑束来在大鼠中建模。对偏侧选择反应时间的评估通常在 9 孔箱中进行，9 孔箱是一种高度专业化的设备，在许多行为实验室中并不广泛使用。另一方面，可伸缩杠杆斯金纳箱更容易获得，并且可能具有更快训练动物的额外优势。
本研究评估了损伤大鼠在可伸缩杠杆斯金纳箱中进行的偏侧选择反应时间任务上的表现，该任务允许对神经退行性疾病模型的行为特征进行定量评估。
在这里，我们比较了接受单侧 6-OHDA 中脑束内侧束损伤的预先训练的雌性 Lister Hooded 大鼠与未处理对照在斯金纳箱中进行的选择反应时间任务的术后表现。
损伤动物在对侧准确性、可用试验次数减少以及对侧反应和双侧运动时间潜伏期减慢方面表现出损伤。
提出的发现允许在实验室之间进行更多的比较，并且可能对该 PD 模型的治疗策略和补救措施的研究有用。
