Department of Biomedical Engineering, University of Minnesota, 7-105 Nils Hasselmo Hall, 312 Church Street SE, Minneapolis, MN 55455, USA.
Microvasc Res. 2013 Nov;90:12-22. doi: 10.1016/j.mvr.2013.07.010. Epub 2013 Aug 9.
This study aimed to form microvessels in fibrin gels, which is of interest both for studying the fundamental cell-matrix interactions as well as for tissue engineering purposes, and to align the microvessels, which would provide natural inlet and outlet sides for perfusion. The data reported here demonstrate the formation of highly interconnected microvessels in fibrin gel under defined medium conditions and the ability to align them using two methods, both of which involved anchoring the gel at both ends to constrain the cell-induced compaction. The first method used only defined medium and resulted in moderate alignment. The second method used defined and serum-containing media sequentially to achieve high levels of microvessel alignment.
本研究旨在在纤维蛋白凝胶中形成微血管，这对于研究基本的细胞-基质相互作用以及组织工程目的都很有意义，并且使微血管排列整齐，为灌注提供自然的入口和出口侧。这里报告的数据表明，在定义的培养基条件下，纤维蛋白凝胶中可以形成高度互联的微血管，并且可以使用两种方法将其排列整齐，这两种方法都涉及将凝胶的两端固定以限制细胞诱导的凝聚。第一种方法仅使用定义的培养基，结果得到适度的排列。第二种方法依次使用定义的培养基和含血清的培养基，以达到高水平的微血管排列。
