Moosavi S Hoda, Gohn-Kreuz Cristian, Rohrbach Alexander
Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering-IMTEK, University of Freiburg, Freiburg, Germany.
Appl Opt. 2013 Aug 10;52(23):5835-42. doi: 10.1364/AO.52.005835.
Confocal line detection has been shown to improve contrast in light-sheet-based microscopy especially when illuminating the sample by Bessel beams. Besides their self-reconstructing capability, the stability in propagation direction of Bessel beams allows to block the unwanted emission light from the Bessel beam's ring system. However, due to phase aberrations induced especially at the border of the specimen, Bessel beams may not propagate along lines parallel to the slit detector. Here we present a concept of how to correct the phase of each incident Bessel beam such that the efficiency of confocal line detection is improved by up to 200%-300%. The applicability of the method is verified by the results obtained from numerical simulations based on the beam propagation method.
共焦线检测已被证明可以提高基于光片显微镜的对比度，特别是在用贝塞尔光束照射样品时。除了具有自重建能力外，贝塞尔光束在传播方向上的稳定性还允许阻挡来自贝塞尔光束环形系统的不需要的发射光。然而，由于特别是在样品边界处引起的相位像差，贝塞尔光束可能不会沿着与狭缝探测器平行的线传播。在这里，我们提出了一种如何校正每个入射贝塞尔光束相位的概念，从而使共焦线检测的效率提高高达200%-300%。基于光束传播方法的数值模拟结果验证了该方法的适用性。
