Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 102, Freiburg 79110, Germany.
Nat Commun. 2012 Jan 17;3:632. doi: 10.1038/ncomms1646.
Laser beams that can self-reconstruct their initial beam profile even in the presence of massive phase perturbations are able to propagate deeper into inhomogeneous media. This ability has crucial advantages for light sheet-based microscopy in thick media, such as cell clusters, embryos, skin or brain tissue or plants, as well as scattering synthetic materials. A ring system around the central intensity maximum of a Bessel beam enables its self-reconstruction, but at the same time illuminates out-of-focus regions and deteriorates image contrast. Here we present a detection method that minimizes the negative effect of the ring system. The beam's propagation stability along one straight line enables the use of a confocal line principle, resulting in a significant increase in image contrast. The axial resolution could be improved by nearly 100% relative to the standard light-sheet techniques using scanned Gaussian beams, while demonstrating self-reconstruction also for high propagation depths.
即使存在大规模的相位扰动，也能够自我重建初始光束轮廓的激光束能够在不均匀介质中更深地传播。这种能力对于基于光片的显微镜技术在厚介质中的应用具有至关重要的优势，例如细胞团、胚胎、皮肤或脑组织或植物，以及散射合成材料。贝塞尔光束的中心强度最大值周围的环形系统使其能够自我重建，但同时也照亮了离焦区域，降低了图像对比度。在这里，我们提出了一种最小化环形系统负面影响的检测方法。光束沿着一条直线的传播稳定性允许使用共焦线原理，从而显著提高图像对比度。与使用扫描高斯光束的标准光片技术相比，轴向分辨率可提高近 100%，同时也证明了在高传播深度下的自我重建能力。
