Johnston C I, Matthews P G, Davis J M, Morgan T
Pflugers Arch. 1975;356(3):277-86. doi: 10.1007/BF00583839.
Plasma renin could be detected in 16 nl of rat plasma by incubating at 50 degrees C, pH 6.2 with 20 mul of a rat renin substrate. In 60 nl samples it could be estimated and differences in concentration of 20% could be detected. Plasma renin concentration was measured in blood from the superficial efferent arterioles of a rat's kidney. The concentration of renin in different efferent arterioles of the same rat were similar. The renin concentration of efferent arteriolar blood was 361 plus or (-1)89 (S.D.) ng Al ml minus 1 (blood) hour (-1) which was not different from the renin concentration of simultaneously taken femoral artery blood (340 +/- 217). Plasma concentration can be measured in efferent arteriolar blood and will allow the control of renin secretion to be studied.
通过在50摄氏度、pH值6.2的条件下，将16纳升大鼠血浆与20微升大鼠肾素底物一起孵育，可检测大鼠血浆中的肾素。在60纳升的样本中，可以对其进行估算，并且能够检测到20%的浓度差异。测定了大鼠肾脏浅表出球小动脉血液中的血浆肾素浓度。同一只大鼠不同出球小动脉中的肾素浓度相似。出球小动脉血液中的肾素浓度为361±189（标准差）纳克铝每毫升血液每小时，这与同时采集的股动脉血液中的肾素浓度（340±217）没有差异。可以测定出球小动脉血液中的血浆浓度，这将有助于研究肾素分泌的控制。
