用于荧光量子产率测量的日蚀热透镜光谱法。
Eclipsing thermal lens spectroscopy for fluorescence quantum yield measurement.
作者信息
Estupiñán-López C, Tolentino Dominguez C, de Araujo R E
机构信息
Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Cidade Universitária, Recife-PE, 50740-530, Brazil.
出版信息
Opt Express. 2013 Jul 29;21(15):18592-601. doi: 10.1364/OE.21.018592.
A modified spatial filtering method that improves the sensitivity of single-beam and mode-mismatched thermal lens spectroscopy (TLS) for fluorescence quantum yield measurement is presented. The method is based on the detection of the external part of a laser beam transmitted by the fluorescent sample (eclipsing detection mode). The experimental results show that the signal/noise (S/N) ratio of the absolute quantum yield of Rh6G can be enhanced up to ~1400% using the eclipsing detection mode on the TLS experimental setup. The method was evaluated by measuring the fluorescence quantum yield of varying concentration of ethanolic solutions of Rhodamine 6G.
提出了一种改进的空间滤波方法，该方法提高了单光束和模式失配热透镜光谱法（TLS）用于荧光量子产率测量的灵敏度。该方法基于对荧光样品透射的激光束外部部分的检测（蚀相检测模式）。实验结果表明，在TLS实验装置上使用蚀相检测模式，罗丹明6G绝对量子产率的信噪比（S/N）可提高至约1400%。通过测量不同浓度的罗丹明6G乙醇溶液的荧光量子产率对该方法进行了评估。
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