[The effect of the method of detecting indocyanine green (HPLC, spectrophotometry) on the determination of pharmacokinetic parameters].

The method of determination of indocyanine green (ICG) in plasma by means of high-performance liquid chromatography (HPLC) is compared with the method of spectrophotometry. A sample for HPLC is purified by deproteination with acetonitrile and the supernatant is directly spread on the column. The internal standard is diazepam. The mobile phase consists of 50% of phosphate buffer pH 6 prepared according to PhBs 4, 47% of acetonitrile and 3% of methanol. Detection takes place at 260 nm. Spectrophotometric analysis consists in the measurement of plasma absorbance at 805 nm. Correlation of both methods is linear r = 0.989, n = 20, p less than 0.05. From the curves of elimination of the dye from the blood bed the principal pharmacokinetic parameters were calculated by means of a one-compartmental model after single intravascular administration. In spectrophotometric examination, elimination of ICG seems to be slower as compared with HPLC.