Liu Hong, Feng Qiao-ling, Zhang Wei, Xie Yong-jun, Li Chao, Jiang Heng, Zhou En-liang
Clinical Medicine School, Chengdu Medical College, Chengdu 610083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2013 Aug 18;45(4):649-53.
To modify the success rate of establishing VX2 transplanted tumor model with different methods in rabbits, and access new typed modification and improved technique in catheterization.
In the study, 30 rabbits were randomly divided into 2 groups. In prophase, tumor cell suspension was implanted in group I, while tumor tissue particles were implanted into liver under direct vision, to establish VX2 transplanted tumor model. The rabbits were catheterized from femoral artery to selective hepatic artery under DSA, by using conventional modification with Seldinger technique in group I and by using new typed modification with improved technique in group II. The imaging and histological features of VX2 tumor were evaluated by combining pathology and DSA, then the success rate, operation time and postoperative complications were compared and evaluated.
The success rates of the liver tumor model were 60.0% and 93.3%; the disposable success rates of catheterization were 66.7% and 92.8%; the operation time of catheterization were (35.6±5.8) min and (27.4±5.3) min; the incidence rates of adverse reaction were 22.5% and 18.0%; the differences between the two groups in the experimental rabbits were significant (P<0.05) statistically.
The efficiency of tumor tissue particles implanted is better than that of tumor cell suspension implanted in establishing VX2 transplanted tumor model under direct vision. The cathetenzation quality and outcomes of new typed modification by improved technique, from femoral artery to selective hepatic artery, is superior to those of conventional modification with Seldinger technique.
采用不同方法改进兔VX2移植瘤模型的建立成功率，并探索新型改良及改进的插管技术。
本研究将30只兔随机分为2组。前期，I组植入肿瘤细胞悬液，II组在直视下将肿瘤组织颗粒植入肝脏，建立VX2移植瘤模型。两组兔均在DSA引导下经股动脉行选择性肝动脉插管，I组采用传统改良Seldinger技术，II组采用新型改良的改进技术。结合病理学和DSA评估VX2肿瘤的影像学和组织学特征，然后比较并评估成功率、手术时间和术后并发症。
肝肿瘤模型的成功率分别为60.0%和93.3%；插管一次性成功率分别为66.7%和92.8%；插管手术时间分别为(35.6±5.8)分钟和(27.4±5.3)分钟；不良反应发生率分别为22.5%和18.0%；两组实验兔之间的差异有统计学意义（P<0.05）。
在直视下建立VX2移植瘤模型时，植入肿瘤组织颗粒的效率优于植入肿瘤细胞悬液。采用改进技术进行新型改良，从股动脉到选择性肝动脉的插管质量和效果优于传统的Seldinger技术改良。
