Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province, 475004, PR China.
Biosens Bioelectron. 2014 Jan 15;51:76-81. doi: 10.1016/j.bios.2013.07.029. Epub 2013 Jul 26.
A novel nanocomposite consisting of graphene nanoplatelets (GNPs) and titanate nanotubes (TNTs) have been synthesized successfully utilizing the hydrothermal method. The GNP-TNT composite was characterized by transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and electrochemical impedance spectroscopy. The voltammetric characterization of GNP-TNT composite, pure GNPs and pure TNTs modified horseradish peroxidase (HRP) biosensors were conducted to select the most suitable electrode immobilization material for enzyme biosensors. The GNPs was firstly eliminated owing to its extremely high background charging current, distinct electrochemical interference from its surface functional groups and low signal to noise ratio. Next, the direct electron transfer of HRP on electrode and the catalytic current of HRP towards H2O2 was increased around 45% and 72% respectively on GNP-TNT composite modified electrodes compared with those on pure TNTs modified electrodes. GNP-TNT composite modified HRP biosensor also exhibited superiority over pure TNTs modified HRP biosensor in the analytical performance. The precision and stability study provided additional evidence for the feasibility of using GNP-TNT composite as electrode modification material.
一种由石墨烯纳米片（GNPs）和钛酸盐纳米管（TNTs）组成的新型纳米复合材料已成功利用水热法合成。通过透射电子显微镜、X 射线衍射、傅里叶变换红外光谱和电化学阻抗谱对 GNP-TNT 复合材料进行了表征。对 GNP-TNT 复合材料、纯 GNPs 和纯 TNTs 修饰辣根过氧化物酶（HRP）生物传感器的伏安特性进行了表征，以选择最适合酶生物传感器的电极固定材料。首先排除了 GNPs，因为其极高的背景充电电流、表面官能团的明显电化学干扰和低信噪比。其次，与纯 TNTs 修饰电极相比，在 GNP-TNT 复合材料修饰电极上，HRP 的直接电子转移和 HRP 对 H2O2 的催化电流分别增加了约 45%和 72%。GNP-TNT 复合材料修饰的 HRP 生物传感器在分析性能方面也优于纯 TNTs 修饰的 HRP 生物传感器。精密性和稳定性研究为使用 GNP-TNT 复合材料作为电极修饰材料的可行性提供了额外的证据。
