Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
Biosens Bioelectron. 2015 Apr 15;66:208-15. doi: 10.1016/j.bios.2014.11.028. Epub 2014 Nov 20.
A significant aspect of this work is the development of a multi-wall carbon nanotube (MWCNT)-titanate nanotube (TNT) nanocomposite to serve as a biocompatible scaffold with high conductivity on a biosensor surface. Unlike other scaffolds consisting of MWCNTs alone or TNTs alone, the MWCNT-TNT nanocomposite synergistically provides excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio. For comparison, after characterising a scaffold consisting of MWCNTs alone, TNTs alone and a MWCNT-TNT nanocomposite using several spectroscopic techniques, the analytical performance of a horseradish peroxidase (HRP) electrochemical biosensor was evaluated using cyclic voltammetry and differential pulse voltammetry. The scaffold consisting of MWCNTs alone displayed a high background charging current, a low signal-to-noise ratio and distinct electrochemical interference from its surface functional groups. In contrast, the direct electrochemistry and the catalytic capability of HRP at MWCNT-TNT modified biosensors towards H2O2 was demonstrated to be ~51% and ~144% enhanced, respectively, compared to those at TNT modified biosensors. Meanwhile, MWCNT-TNT nanocomposite modified HRP biosensors also exhibited higher sensitivity (4.42μAmM(-1)) than TNT modified HRP biosensors (1.48μAmM(-1)). The above superior performance was attributed to the improved properties of MWCNT-TNT nanocomposite as biosensor scaffold compared to its two individual components by complementing each component and synergistically sustaining the characteristic features of each component.
这项工作的一个重要方面是开发一种多壁碳纳米管（MWCNT）-钛酸盐纳米管（TNT）纳米复合材料，作为生物传感器表面具有高导电性的生物相容性支架。与由单独的 MWCNT 或 TNT 组成的其他支架不同，MWCNT-TNT 纳米复合材料协同提供了优异的生物相容性、良好的导电性、低电化学干扰和高信噪比。为了进行比较，在用几种光谱技术对由单独的 MWCNT、单独的 TNT 和 MWCNT-TNT 纳米复合材料组成的支架进行了表征之后，使用循环伏安法和差分脉冲伏安法评估了辣根过氧化物酶（HRP）电化学生物传感器的分析性能。由单独的 MWCNT 组成的支架显示出高背景充电电流、低信噪比和来自其表面官能团的明显电化学干扰。相比之下，与在 TNT 修饰的生物传感器相比，在 MWCNT-TNT 修饰的生物传感器中，HRP 的直接电化学和对 H2O2 的催化能力分别提高了约 51%和 144%。同时，MWCNT-TNT 纳米复合材料修饰的 HRP 生物传感器也表现出比 TNT 修饰的 HRP 生物传感器更高的灵敏度（4.42μAmM(-1))（1.48μAmM(-1))。与两个单独的组件相比，MWCNT-TNT 纳米复合材料作为生物传感器支架具有改善的性能，通过互补每个组件并协同维持每个组件的特征，从而导致了上述优越的性能。
