Veterinary Integrated Research Unit, Faculty of Sciences, Namur Research Institute for Life Sciences, University of Namur, 5000 Namur, Belgium.
Friedrich-Loeffler-Institut, Greifswald-Insel-Riems, Germany.
J Gen Virol. 2013 Nov;94(Pt 11):2399-2405. doi: 10.1099/vir.0.055954-0. Epub 2013 Aug 12.
Schmallenberg virus (SBV) is an emerging arbovirus infecting ruminants in Europe. SBV belongs to the Bunyaviridae family within the Simbu serogroup. Its genome comprises three segments, small (S), medium (M) and large (L), that together encode six proteins and contain NTRs. NTRs are involved in initiation and termination of transcription and in genome packaging. This study explored the 3' mRNA termini of SBV and related Simbuviruses. In addition, the 5' termini of SBV messenger RNA (mRNA) were characterized. For the three SBV segments, cap-snatching was found to initiate mRNA transcription both in vivo and in vitro. The presence of extraneous nucleotides between host RNA leaders and the viral termini fits with the previously described prime-and-realign theory. At the 3' termini, common features were identified for SBV and related Simbuviruses. However, different patterns were observed for the termini of the three segments from the same virus type.
沙氏门菌病毒（SBV）是一种新兴的感染欧洲反刍动物的虫媒病毒。SBV 属于布尼亚病毒科辛布病毒属。其基因组由三个节段组成，小（S）、中（M）和大（L），共同编码六种蛋白，并包含 NTRs。NTRs 参与转录的起始和终止以及基因组包装。本研究探索了 SBV 和相关辛布病毒的 3'mRNA 末端。此外，还对 SBV 信使 RNA（mRNA）的 5'末端进行了表征。对于三个 SBV 片段，体内和体外实验均发现帽捕捉起始了 mRNA 转录。宿主 RNA 前导序列和病毒末端之间存在外源核苷酸，这与之前描述的引物和重排理论相符。在 3'末端，发现 SBV 和相关辛布病毒具有共同特征。然而，同一病毒类型的三个片段的末端观察到不同的模式。
