Spitzer R E, Stitzel A E, Tsokos G C
Department of Pediatrics, State University of New York, Health Science Center, Syracuse 13210.
Clin Immunol Immunopathol. 1990 Oct;57(1):19-31. doi: 10.1016/0090-1229(90)90019-m.
Anti-idiotypic antibodies to autoantibody against the alternative pathway C3 convertase (C3NeF) were isolated and purified from normal human serum as well as from serum from six patients with membrano-proliferative glomerulonephritis (MPGN). All preparations of anti-id antibody blocked C3NeF deposition on EC3bBb as well as C3NeF stabilization of EC3bBb functional activity. The Ka of these ant-id antibodies for C3NeF was 10(9) liters/mol which is comparable to the Ka of C3NeF for its antigen. In addition, 90% of anti-id antibody isolated from patients with MPGN and 20% isolated from normal individuals resembled Bb and bound to C3b as well as to antibody specific for the Bb portion of Factor B. These anti-id antibodies also resembled C3b and bound to antibody specific for the C3c portion of C3b. Immunization of rabbits with this latter form of anti-id antibody led to the production of functionally active C3NeF. These data indicate that C3NeF anti-idiotypic antibodies exist in two distinct forms, with and without internal imagery of C3bBb, and can occur in both normal individuals and patients with MPGN.
从正常人血清以及六名膜增生性肾小球肾炎（MPGN）患者的血清中分离并纯化了针对替代途径C3转化酶自身抗体（C3NeF）的抗独特型抗体。所有抗独特型抗体制剂均能阻断C3NeF在EC3bBb上的沉积以及EC3bBb功能活性的C3NeF稳定作用。这些抗独特型抗体对C3NeF的Ka为10（9）升/摩尔，这与C3NeF对其抗原的Ka相当。此外，从MPGN患者中分离出的90%抗独特型抗体和从正常个体中分离出的20%抗独特型抗体类似于Bb，并与C3b以及针对B因子Bb部分的抗体结合。这些抗独特型抗体也类似于C3b，并与针对C3b C3c部分的抗体结合。用后一种形式的抗独特型抗体免疫兔子会导致产生功能活性的C3NeF。这些数据表明，C3NeF抗独特型抗体以两种不同形式存在，具有和不具有C3bBb的内影像，并且可存在于正常个体和MPGN患者中。
