Tsokos G C, Stitzel A E, Patel A D, Hiramatsu M, Balow J E, Spitzer R E
Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20892.
Clin Immunol Immunopathol. 1989 Oct;53(1):113-22. doi: 10.1016/0090-1229(89)90106-2.
Complement 3 nephritic factors (C3NeF) were isolated from the sera of patients with membranoproliferative glomerulonephritis (MPGN) and the supernatants of pokeweed mitogen-stimulated mononuclear cells from patients with MPGN. Three human monoclonal C3NeF antibodies (two IgGs, CK and PH, and one IgM, K3C4) were established. Using an exhaustive series of affinity columns, we isolated anti-C3NeF idiotypic antibodies (anti-IdNeF) (three from normal and two from patient sera). Anti-IdNeF preparations bound to F(ab')2-NeF and prevented its ability to stabilize C3bBb convertase. We have used the above reagents to address questions on the genesis and the diversity of C3NeF antibodies. The following results were obtained: All anti-IdNeF preparations bound to C3NeF isolated from patient sera, cell culture supernatants, and IgG and IgM monoclonal C3NeF. None of the monoclonal C3NeF bound to an extensive battery of common antigens, including Fc portion of IgG, TNP, beta-galactosidase, DNA, and bacterial products. These data indicate that C3NeF express one common idiotype and that these antibodies are not raised in response to an obvious antigen.
从膜增生性肾小球肾炎（MPGN）患者的血清以及MPGN患者经商陆有丝分裂原刺激的单核细胞上清液中分离出补体3肾炎因子（C3NeF）。建立了三种人源单克隆C3NeF抗体（两种IgG，即CK和PH，以及一种IgM，即K3C4）。通过一系列详尽的亲和柱，我们分离出了抗C3NeF独特型抗体（抗IdNeF）（三种来自正常人血清，两种来自患者血清）。抗IdNeF制剂与F(ab')2-NeF结合，并阻止其稳定C3bBb转化酶的能力。我们使用上述试剂来探讨关于C3NeF抗体的起源和多样性问题。获得了以下结果：所有抗IdNeF制剂均与从患者血清、细胞培养上清液以及IgG和IgM单克隆C3NeF中分离出的C3NeF结合。没有一种单克隆C3NeF与大量常见抗原结合，包括IgG的Fc部分、三硝基苯（TNP）、β-半乳糖苷酶、DNA和细菌产物。这些数据表明C3NeF表达一种共同的独特型，并且这些抗体不是针对明显抗原产生的。
