Sun Feng-Xian, Wang Man, Xu Yan-Ling, Lin Lai-Xiang, Xu Shu-Mei
Department of Physiology, Tianjin Medical University, Tianjin 300070, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2013 May;29(3):239-44.
To study the effect of combining the injection of beta-sheet breaker H102 with human umbilical cord mesenchymal stem cell (hUCMSC) on APP transgenic mice behavior, P-tau, apoptosis and the expression of relevant enzymes in the brain.
APP transgenic mice were randomly divided into model group, hUCMSC group, H102 group, H102 with hUCMSC group and a group of C57BL/6J mice with the same age and background was set as normal. After two weeks and four weeks, the ability of spatial reference memory was tested by Morris Water Maze. After four weeks, immunohistochemical stain and Western blot were done to detect the content of Bad, Bax, Bcl-2, Bcl-xl, P-tau, GSK-3beta, PP-2A and PP-1 in mice brain.
The ability of memory of hUCMSC in 2 weeks group was slightly improved than that in the model group. hUCMSC in four weeks group, H102 group and H102 with hUCMSC group significantly improved the ability of and memory, and reduced the phosphorylation of tau and brain cell's apoptosis of the Alzheimer disease (AD) mice.
Beta-sheet breaker H102 together with transplanting hUCMSC is an effective therapeutic strategy for AD.
研究β-折叠破坏肽H102与人脐带间充质干细胞（hUCMSC）联合注射对APP转基因小鼠行为、P- tau蛋白、细胞凋亡及脑内相关酶表达的影响。
将APP转基因小鼠随机分为模型组、hUCMSC组、H102组、H102与hUCMSC联合组，并设同龄同背景的C57BL/6J小鼠为正常组。两周和四周后，通过Morris水迷宫检测空间参考记忆能力。四周后，进行免疫组织化学染色和蛋白质免疫印迹法检测小鼠脑内Bad、Bax、Bcl-2、Bcl-xl、P- tau、GSK-3β、PP-2A和PP-1的含量。
2周龄hUCMSC组小鼠的记忆能力较模型组略有改善。4周龄hUCMSC组、H102组及H102与hUCMSC联合组显著改善了阿尔茨海默病（AD）小鼠的记忆能力，降低了tau蛋白磷酸化水平及脑细胞凋亡。
β-折叠破坏肽H102与移植hUCMSC联合应用是治疗AD的有效策略。
