Shindo S
Department of Forensic Medicine, Akita University School of Medicine, Japan.
Electrophoresis. 1990 Jun;11(6):483-8. doi: 10.1002/elps.1150110609.
Specific antibodies against the human haptoglobin (HP) alpha chain were raised and used for immunoblotting after isoelectric focusing to determine Hp alpha subtypes in a reproducible and simplified manner. By eliminating the staining of the HP beta chain, HP alpha subtypes were visualized more precisely and simply than by previously reported methods. Subtypes of a total of 1211 sera, obtained from two different populations in Japan, were examined by the new method. Four HP alpha subtypes (HP 1S-1S,2FS-1S, 2FS-2FS and 2FS-2SS) and five subtypes combined with a new variant (HP 2FS-1V1, 2V1-1S, 2FS-2V1, 2FS-2V2 and 2FS-2V3) were observed. HP 1V1 belonged to HP alpha 1, and HP 2V1, 2V2 and 2V3 belonged to HP alpha 2, and their alleles were designated HP A1V1, HP A2V1, HP A2V2 and HP A2V3, respectively. The allele frequencies were calculated to be as follows: HP A1S, 0.2597; HP A1V1, 0.0004; HP A2FS, 0.7333; HP A2SS, 0.0008; HP A2V1, 0.0045; HP A2V2, 0.0008; and HP A2V3, 0.0004. The allele frequency of HP A2SS, which is common in the European population, is less frequent than HP A*2V1 in the Japanese population.
制备了针对人触珠蛋白（HP）α链的特异性抗体，并在等电聚焦后用于免疫印迹，以一种可重复且简化的方式确定Hpα亚型。通过消除HPβ链的染色，与先前报道的方法相比，HPα亚型的可视化更加精确和简单。采用新方法检测了从日本两个不同人群获得的总共1211份血清的亚型。观察到四种HPα亚型（HP 1S-1S、2FS-1S、2FS-2FS和2FS-2SS）以及五种与新变体组合的亚型（HP 2FS-1V1、2V1-1S、2FS-2V1、2FS-2V2和2FS-2V3）。HP 1V1属于HPα1，HP 2V1、2V2和2V3属于HPα2，它们的等位基因分别命名为HP A1V1、HP A2V1、HP A2V2和HP A2V3。计算得到的等位基因频率如下：HP A1S，0.2597；HP A1V1，0.0004；HP A2FS，0.7333；HP A2SS，0.0008；HP A2V1，0.0045；HP A2V2，0.0008；HP A2V3，0.0004。在欧洲人群中常见的HP A2SS的等位基因频率在日本人群中比HP A*2V1的频率更低。
