Nakada N, Abe K
Department of Legal Medicine, Tokyo Women's Medical College, Japan.
Hum Hered. 1987;37(6):376-80. doi: 10.1159/000153738.
Haptoglobin (Hp) subtypes have been determined in the Japanese population by polyacrylamide gel isoelectric focusing followed by immunoblotting and by two-dimensional polyacrylamide gel electrophoresis. In the present study, neuraminidase-treated plasma samples were used for subtyping of Hp, without prior purification. These samples were obtained from 372 unrelated healthy donors. Allelic frequencies were: Hp1F = 0.0014; Hp1S+ = 0.2688; Hp2FF = 0.0000; Hp2FS = 0.7284, and Hp*2SS = 0.0014. The phenotypic distribution was in good accordance with the Hardy-Weinberg equilibrium.
通过聚丙烯酰胺凝胶等电聚焦后免疫印迹法以及二维聚丙烯酰胺凝胶电泳法，已在日本人群中确定了触珠蛋白（Hp）亚型。在本研究中，未经预先纯化的神经氨酸酶处理血浆样本被用于Hp亚型分析。这些样本取自372名无亲缘关系的健康供者。等位基因频率分别为：Hp1F = 0.0014；Hp1S+ = 0.2688；Hp2FF = 0.0000；Hp2FS = 0.7284，以及Hp*2SS = 0.0014。表型分布符合哈迪-温伯格平衡。
