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通过聚丙烯酰胺凝胶等电聚焦分析6-磷酸葡萄糖酸脱氢酶同工酶。

Analysis of 6-phosphogluconate dehydrogenase isozymes by polyacrylamide gel isoelectric focusing.

作者信息

Akiyama K

机构信息

Department of Legal Medicine, Tokyo Women's Medical College, Japan.

出版信息

Electrophoresis. 1990 Jun;11(6):509-10. doi: 10.1002/elps.1150110615.

DOI:10.1002/elps.1150110615
PMID:2394215
Abstract

Three common phenotypes of 6-phosphogluconate dehydrogenase (6-PGD) were identified by enzyme visualization following isoelectric focusing in photochemically polymerized polyacrylamide gels whereas in chemically polymerized gels no activity could be detected. Gene frequencies calculated from 429 individuals were 0.915 and 0.085 for 6-PGDA and 6-PGDC, respectively, similar to the gene frequencies of Japanese samples previously reported.

摘要

通过在光化学聚合的聚丙烯酰胺凝胶中进行等电聚焦后进行酶可视化，鉴定出6-磷酸葡萄糖酸脱氢酶（6-PGD）的三种常见表型，而在化学聚合的凝胶中未检测到活性。根据429名个体计算出的6-PGDA和6-PGDC基因频率分别为0.915和0.085，与先前报道的日本样本的基因频率相似。

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